Chlamydophila / Mycoplasma felis PCR Detection kit
(Real time qPCR fluorescent probe method)
Intended use:
This kit is suitable for the specific detection of Chlamydophila felis (CF) and Mycoplasma felis (MF) in cat eye nasal and mouth secretions.
Principle:
This kit selection of chlamydia and mycoplasma conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, Fluorescence was emitted and detected by fluorescence PCR instrument to realize the detection of chlamydia and mycoplasma nucleic acids in the completely closed reaction system.
Main components:
| Component | Specification |
| PCR reaction liquid | 8 tubes |
| Positive control | 1 tube |
| Negative control | 1 tube |
Note: components in different batches of kits should not be interchangeable.
Sample requirements:
1. Sample type
Cat eye, mouth and nose secretion and other samples
2. Sample collection and transportation
Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.
PCR amplification and fluorescence Detection (PCR detection area)
Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:
| Step | Cycle number | Temperature | Time |
| 1 | 1 | 95℃ | 3min |
| 2 | 45 | 95℃ | 10s |
| 60℃ | 30s collect fluorescent |
FAM and VIC/HEX are selected for detection channels.
The results of interpretation
| | FAM channel (CF) | VIC/HEX channel (MF) | Result interpretation |
| 1 | - | - | Results were reported negative |
| 2 | + | - | Results were reported CF positive |
| 3 | - | + | Results were reported MF positive |
| 4 | + | + | Results were reported CF,MF positive |
Positive judgment value or reference interval
| Result | Ct Value |
| Positive | Ct≤38 |
| Negative | No Ct value or Ct value=0 |
| Gray area | 38＜Ct≤45 |
Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
Operation details please check from IFU!
Product Tags:
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DNA Taq Feline Mycoplasma PCR Kit Chlamydophila Real Time Fluorescent Probe PCR System Images
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